Left: Images of fluorescent particles that are above, at and below (top to bottom) the vertical position of best focus of a microscope. Calibrating the effects of lens aberrations on the apparent ...

New work enables optical microscopes to measure these nanometer-scale details with a new level of accuracy. Over the last two decades, scientists have discovered that the optical microscope can be ...

Breakthroughs, discoveries, and DIY tips sent every weekday. Terms of Service and Privacy Policy. Today, we take for granted the instruments that let us look at tiny ...

(Nanowerk Spotlight) The drawbacks of existing measures for calibrating scanning probe microscopes (SPMs) based on various diffraction gratings and periodic ...

Zooming in: image of mouse embryo. (Courtesy: Gail McConnell/University of Strathclyde) A new microscope lens that offers the unique combination of a large field of view with high resolution has been ...

Optical aberrations and the errors they introduce are a standard obstacle in localization microscopy techniques, and hinder the gathering of depth information from a biological sample. A project at ...

It’s relatively easy to understand how optical microscopes work at low magnifications: one lens magnifies an image, the next magnifies the already-magnified image, and so on until it reaches the eye ...