NanoDrop One Spectrophotometer finds and fixes DNA/RNA contamination - Scientific Videos | Thermo Fisher Scientific US
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SOLVED: 7. Quantification of DNA can be done by using aNanodrop, a UV spectrophotometer, by measuring its absorbance inunits of optical density (OD) (see “Nanodrop MicrovolumeQuantitation of Nucleic Acids” video in Lab Activity Videos for Lab3 on Laulima for more information). DNA absorbs light most stronglyat the ultraviolet wavelength of 260 nm. The absorbance ofdouble stranded DNA (dsDNA) at 260 nm(A260) is used toestimate concentration, with 1.0 OD equal to a dsDNA concentrationof 50 μg/ml
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